DSIP reconstitution: water for 5 and 10 mg vials, the mcg/mL and mcg per unit each gives, what the record says about DSIP in solution, and FDA's status note.
DSIP is sold as a powder in 5 mg and 10 mg vials and is discussed in microgram amounts, which makes its reconstitution question mostly a readability question: how much water spreads the powder so that a small amount shows up as more than a unit or two on the syringe. No DSIP product has a label, so nothing official answers it. What can be set out is the arithmetic for each water volume, what the literature reports about DSIP in solution, and what the volumes look like against the trial amounts, which were much larger and all intravenous (sources opened 2026-10-20 unless dated otherwise). This page reports; it suggests no amount and no water volume.
The trial doses themselves, converted from nanomoles per kilogram, and the units for the circulating figures are on the DSIP dosage page. Any other vial and volume is worked by the DSIP vial calculator; every compound is indexed on the reconstitution hub.
Water per vial and the concentration it gives
| Vial |
Water |
Concentration |
mcg per U-100 unit |
100 mcg |
300 mcg |
| 5 mg |
1 mL |
5,000 mcg/mL |
50 |
2 units |
6 units |
| 5 mg |
2 mL |
2,500 mcg/mL |
25 |
4 |
12 |
| 5 mg |
2.5 mL |
2,000 mcg/mL |
20 |
5 |
15 |
| 5 mg |
3 mL |
1,667 mcg/mL |
16.7 |
6 |
18 |
| 5 mg |
5 mL |
1,000 mcg/mL |
10 |
10 |
30 |
| 10 mg |
1 mL |
10,000 mcg/mL |
100 |
1 |
3 |
| 10 mg |
2 mL |
5,000 mcg/mL |
50 |
2 |
6 |
| 10 mg |
2.5 mL |
4,000 mcg/mL |
40 |
2.5 |
7.5 |
| 10 mg |
3 mL |
3,333 mcg/mL |
33.3 |
3 |
9 |
| 10 mg |
5 mL |
2,000 mcg/mL |
20 |
5 |
15 |
One U-100 unit is 0.01 mL. The 100 and 300 mcg columns are the range circulating on vendor pages for the vial, as recorded on this site's dosage chart on 2026-09-18; they are not trial doses. The two columns show the vial's practical problem: at 1 mL, a 10 mg vial puts 100 mcg on a single unit mark, so a reading error of half a unit is half the amount. Every extra millilitre of water moves the same amount further up the barrel. The 5 mL rows assume a vial with room for that volume and are shown for the arithmetic; the insulin syringe units page shows how 30-, 50- and 100-unit barrels read.
The trial amounts are a different scale
Every human DSIP study gave it intravenously in nanomoles per kilogram — 25 nmol/kg in the sleep studies, up to 100 nmol/kg in a 2009 anaesthesia study — which at DSIP's molecular weight of 848.8 is milligrams, not micrograms. Set against the vials, those amounts change the water question from readability to capacity.
| Trial amount (25 nmol/kg) |
Amount |
Most water keeping it in one 100-unit syringe, 5 mg vial |
Same, 10 mg vial |
Amounts per 5 mg vial |
| 60 kg adult |
1.27 mg |
3.9 mL |
7.9 mL |
3.9 |
| 70 kg adult |
1.49 mg |
3.4 mL |
6.7 mL |
3.4 |
| 90 kg adult |
1.91 mg |
2.6 mL |
5.2 mL |
2.6 |
Formula: maximum water (mL) = vial mg ÷ amount mg × 1 mL. These are ceilings for fitting one draw on one barrel, worked from published intravenous figures, and none of them describes a subcutaneous dose; no published human study gave DSIP under the skin.
How many amounts a vial holds
The water decides how each amount reads on the syringe; it does not change how many amounts the powder holds. That count depends only on the vial size and the amount, and it differs by more than an order of magnitude between the circulating figures and the published ones.
| Amount |
Where it comes from |
5 mg vial |
10 mg vial |
| 100 mcg |
Low end of the circulating range |
50 |
100 |
| 300 mcg |
High end of the circulating range |
16.7 |
33.3 |
| 1.49 mg |
25 nmol/kg at 70 kg, intravenous trials |
3.4 |
6.7 |
| 3 mg |
1995 endocrine study, total infusion (PMID 7777652) |
1.7 |
3.3 |
A vial reconstituted for one of these scales is awkward for the other. Water chosen so that 100 mcg reads as 5 units (2.5 mL in a 5 mg vial) puts 1.49 mg at 74.5 units; water chosen to keep 1.49 mg inside one barrel (3.4 mL at most) puts 100 mcg at about 7 units. Because a reconstituted vial holds one concentration until it is used up, the volume settles the readable range for every draw that follows. The counts are arithmetic on the vial labels and the published figures; none is a course.
What the record says about DSIP in solution
No study of DSIP kept in bacteriostatic water was found, and no label exists to state a shelf life. Two laboratory studies say something about how the molecule behaves in liquid and near tissue:
| Study |
Setting |
Finding |
| Chiang et al., Drug Dev Ind Pharm 1998 (PMID 9876605) |
Buffer solutions; skin, during transdermal delivery |
"Fairly stable in pH 4-9 buffer solutions" but cleaved by skin enzymes, metalloproteases most likely |
| Augustijns and Borchardt, Drug Metab Dispos 1995 (PMID 8689946) |
Human intestinal (Caco-2) cell layers, two hours |
8.2% remained on the gut side and 70.6% on the other; tryptophan was the main breakdown product |
The two findings point in the same direction: the nine-residue chain holds together in a plain buffer and comes apart quickly where enzymes are present. Neither study tested a reconstituted vial, a refrigerator or bacteriostatic water's benzyl alcohol, and neither result should be read as a storage time. What labels and manufacturers say about storing mixed peptides in general is on Medibact's how to store peptides page, and the mixing steps themselves on its how to reconstitute peptides page.
FDA's note on emideltide
FDA's compounding records use the name emideltide. Its page on bulk substances that may present significant safety risks, current as of 2026-04-22, lists emideltide (DSIP) in the table of substances whose nominations were withdrawn and records that compounded emideltide "may pose risk for immunogenicity for certain routes of administration", may have "complexities with regard to peptide-related impurities and API characterization", and that the agency "lacks sufficient information to know whether the drug would cause harm if administered to humans". The page states no dose and no route it considers safe.
What this page does not do
It does not state an amount of DSIP, a water volume to use or a storage time. It works the concentration for each water volume, sets the published intravenous amounts against the vial sizes, and reports the two studies of DSIP in solution and FDA's statement. Medibact's DSIP guide covers the compound. Decisions about administering anything belong with a clinician.
Sources and dates
Opened 2026-10-20: PubMed search for delta sleep-inducing peptide with degradation, stability, half-life or aminopeptidase in title or abstract (30 records), with the abstracts of PMIDs 9876605 and 8689946 read; FDA, "Certain bulk drug substances for use in compounding that may present significant safety risks" (content current as of 2026-04-22). From this site's DSIP dosage page (read 2026-09-19) and the DSIP calculator notes (read 2026-09-24): the molecular weight (PubChem CID 68816), the trial doses and routes, and the DailyMed and ClinicalTrials.gov searches. The circulating 100 to 300 mcg figures are as recorded on this site's dosage chart on 2026-09-18. Concentration, unit and volume arithmetic by this site. Corrections go to the contact page.
