NAD+ Reconstitution: Water per Vial, mg/mL and Units

How 100 mg to 1,000 mg NAD+ vials convert to mg/mL and U-100 units, and why the arithmetic sits an order of magnitude above a peptide vial's, with sources.

Medibact Guides · Published 2026-09-18

Illustration: A digital scale, a clear glass vial, and a pipette precisely arranged on a white surface. — Illustration

NAD+, nicotinamide adenine dinucleotide, is a coenzyme found in every living cell, made of two nucleotides joined through a phosphate bridge. It is not a peptide. It is on this site because it is sold as a lyophilized powder for injection through the same channels as research peptides, in vials of 100 mg, 500 mg, 750 mg and 1,000 mg, the sizes the NAD+ calculator carries. This page reports the water volumes the sources state for those vials, the concentrations they produce, and what the milligram amounts in circulation come to on a U-100 syringe. It reports and does not recommend. The figures and their provenance are on the NAD+ dosage page; every compound's arithmetic is indexed on the reconstitution hub.

The arithmetic is an order of magnitude apart

Every reconstitution page on this site uses the same formula: concentration is the vial mass divided by the water volume, and one U-100 unit is a hundredth of a millilitre. What is different here is the size of every number in it.

	A 10 mg peptide vial in 2 mL	A 500 mg NAD+ vial in 2 mL
Concentration	5 mg/mL	250 mg/mL
In one U-100 unit	50 mcg	2,500 mcg (2.5 mg)
A typical stated amount	200 mcg	100 mg
That amount as a draw	4 units	40 units

The peptide is drawn in a few units; the NAD+ amount in circulation is a few hundred times larger, and the sources answer that not by drawing forty units of a 250 mg/mL solution but by adding far more water, so that the concentration falls to 50 to 200 mg/mL and the draw becomes a large fraction of a syringe or more than one. That is why the water volumes below run from 2 mL to 10 mL rather than the 1 to 3 mL a peptide vial gets.

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What the sources state

Source	What it states	Kind of source	Opened
PubChem, CID 5892	C21H27N7O14P2, molecular weight 663.4	Chemical database	2026-09-18
DailyMed searches	"nicotinamide adenine dinucleotide": zero labels; "NAD+": 93 records, all NADH oral products or homeopathic "Nadidum" preparations, none an injection	Regulator database	2026-09-18
NCT06919328, Nutraceuticals Research Institute	"100mg NAD+ in 2ml bacteriostatic water" by intramuscular, subcutaneous or intravenous-push injection; 70 planned; recruiting; no results	Trial registry	2026-09-18
NCT06382688, the same sponsor	500 mg NAD+ intravenously in 500 mL of saline; 53 participants; status unknown; no results	Trial registry	2026-09-18
Grant et al., Front Aging Neurosci 2019	3 µmol/min intravenously for 6 hours; no plasma change for 2 hours; "rapidly and completely removed from the plasma"	Peer-reviewed pilot pharmacokinetic study	2026-09-18
Yu et al., Am J Cardiovasc Drugs 2026	10 mg/day intravenously for 7 days, 180 adults with heart failure; ejection fraction 45.44% vs 42.44% at one month	Peer-reviewed randomized trial	2026-09-18
Peptide Dosing Protocols, NAD+ guide	500 mg in 5 mL (100 mg/mL) or 2.5 mL (200 mg/mL); 750 mg in 7.5 mL; 1,000 mg in 10 mL or 5 mL; subcutaneous 50 to 100 mg two to three times a week; unbuffered NAD+ at about pH 3 to 4; "Direct human evidence for SubQ NAD+ schedules is limited"	Protocol site, last updated August 2026	2026-09-18

The 716 mg figure for the infusion study is this page's arithmetic, not the paper's: 3 µmol per minute for 360 minutes is 1,080 µmol, and at 663.4 g/mol that is 716 mg. The paper reports a rate.

Water per vial and the concentration it gives

The concentrations the sources use are 50, 100 and 200 mg/mL. The table gives the water that produces each from the calculator's four vial sizes, with the calculator's own 1, 2 and 3 mL columns for the 100 mg vial, the one size at which those volumes land in the same range.

Vial	Water added	Concentration	Milligrams per unit
100 mg	1 mL	100 mg/mL	1 mg
100 mg	2 mL	50 mg/mL	0.5 mg
100 mg	3 mL	33.3 mg/mL	0.33 mg
500 mg	2.5 mL	200 mg/mL	2 mg
500 mg	5 mL	100 mg/mL	1 mg
500 mg	10 mL	50 mg/mL	0.5 mg
750 mg	3.75 mL	200 mg/mL	2 mg
750 mg	7.5 mL	100 mg/mL	1 mg
1,000 mg	5 mL	200 mg/mL	2 mg
1,000 mg	10 mL	100 mg/mL	1 mg

The 100 mg in 2 mL row is the configuration the US registration specifies. The rest are the protocol page's examples or the same concentrations applied to the other vial sizes. One thing the table makes plain: a 1,000 mg vial in 10 mL and a 500 mg vial in 5 mL are the same solution. Doubling the vial and doubling the water changes how many draws the vial holds and nothing about any single draw.

The stated milligram amounts in syringe units

The amounts the sources state are 50 mg and 100 mg subcutaneously (protocol page), 100 mg by injection (the registration), and larger intravenous quantities that are not syringe draws at all. On a U-100 syringe, 100 units is 1 mL.

Concentration	50 mg	100 mg	200 mg
200 mg/mL	25 units (0.25 mL)	50 units (0.5 mL)	100 units (1 mL)
100 mg/mL	50 units (0.5 mL)	100 units (1 mL)	2 mL, two barrels
50 mg/mL	100 units (1 mL)	2 mL, two barrels	4 mL
33.3 mg/mL	1.5 mL	3 mL	6 mL

Two consequences follow. At 50 mg/mL, the registration's concentration, a 100 mg amount is a 2 mL injection, which is why that record describes intramuscular and intravenous routes alongside subcutaneous. And the number of stated amounts in a vial does not depend on the water: a 500 mg vial holds five 100 mg amounts or ten 50 mg amounts at any concentration, because the water changes the draw and not the contents.

The mixing steps, as a lyophilized-injectable leaflet describes them

No NAD+ label exists to quote. The registration record states only the diluent and volume, "100mg NAD+ in 2ml bacteriostatic water". For the procedure itself, the closest labelled description of reconstituting a large-mass lyophilized injectable is the Italian patient leaflet for TAD 600 (a 600 mg powder with 4 mL of water for injection, published by the Italian medicines agency on 2021-04-29): the water is drawn from its ampoule with a syringe, the vial's stopper is disinfected, the needle is inserted through the centre of the stopper and the stream of water is directed against the glass wall of the vial, the vial is agitated gently to complete dissolution, and the solution is used only if clear and free of visible particles. The protocol page's own caution belongs beside that: an unbuffered NAD+ powder dissolves to an acidic solution, about pH 3 to 4, which it associates with stinging at the injection site. The general procedure for any lyophilized vial is on Medibact's how to reconstitute peptides page and is not repeated here.

Storage after mixing

No product label exists for injectable NAD+, so there is no label statement on the solution's storage to report; what labels and manufacturers state for reconstituted vials is on Medibact's how to store peptides page.

What this page does not do

It does not state an amount of NAD+ to inject, a route, a frequency or a course. The 50 mg and 100 mg figures appear because a protocol page and a trial registration state them, and the protocol page says itself that the human evidence for subcutaneous schedules is limited; the 10 mg, 500 mg and 716 mg figures appear because they are what the published and registered studies used, by intravenous routes and in patient groups that have nothing to do with a research vial. The arithmetic converts stated inputs exactly and is silent on whether any input is appropriate. Medibact's NAD+ guide covers the compound; Peptifact's NAD+ dosage page examines the fifty-fold gap between the trial doses and the wellness doses. Decisions about administering anything to a person belong with a clinician.

Sources and dates

Opened 2026-09-18: PubChem's PUG REST record for CID 5892; the DailyMed SPL search API for "nicotinamide adenine dinucleotide" (zero records) and "NAD+" (93 records, titles reviewed); the ClinicalTrials.gov v2 API records NCT06919328 and NCT06382688; PubMed through the NCBI E-utilities for the abstracts of PMID 31572171 (Grant et al. 2019, doi:10.3389/fnagi.2019.00257) and PMID 40954388 (Yu et al. 2026, doi:10.1007/s40256-025-00764-7); the Peptide Dosing Protocols NAD+ guide (last updated August 2026); the AIFA patient leaflet for TAD 600 mg/4 ml (Biomedica Foscama, 2021-04-29); MedlinePlus, "Giving an insulin injection" (reviewed 2024-07-21) for the U-100 scale. Corrections go to the contact page.

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Medibact Guides facts

NAD+ is not a peptide: nicotinamide adenine dinucleotide is a dinucleotide coenzyme, C21H27N7O14P2, molecular weight 663.4 (PubChem CID 5892, opened 2026-09-18). It is catalogued here because it is sold as a lyophilized injectable through the same channels as research peptides.
Injectable NAD+ is sold in 100 mg, 500 mg, 750 mg and 1,000 mg vials, the sizes the calculator carries. A research peptide vial is typically 5 mg or 10 mg, so at the same water volume the NAD+ concentration is 10 to 200 times higher and one U-100 unit carries milligrams rather than tens of micrograms.
DailyMed returned zero labels for 'nicotinamide adenine dinucleotide' on 2026-09-18; the 93 records returned for 'NAD+' are NADH-containing oral products and homeopathic preparations listing 'Nadidum', none an NAD+ injection, so no label states a diluent volume, a concentration or a dose.
A US registration, NCT06919328 (Nutraceuticals Research Institute, 70 planned participants, recruiting, no results as of 2026-09-18), specifies '100mg NAD+ in 2ml bacteriostatic water' by intramuscular, subcutaneous or intravenous-push injection, which is 50 mg/mL and a 2 mL draw; a second, NCT06382688 (53 participants, status unknown), gives 500 mg intravenously in 500 mL of saline.
The one published pharmacokinetic study of infused NAD+ gave 3 µmol per minute intravenously for 6 hours and found no change in plasma NAD+ or its metabolites for the first two hours (Grant et al., Frontiers in Aging Neuroscience 2019). At a molecular weight of 663.4 that rate comes to about 716 mg over the infusion, a conversion this page makes and the paper does not.
The largest randomized placebo-controlled injection trial located gave 10 mg of NAD+ intravenously once a day for 7 days to 180 adults with heart failure from ischaemic cardiomyopathy; ejection fraction at one month was 45.44% against 42.44% for placebo, p = 0.024 (Yu et al., American Journal of Cardiovascular Drugs 2026).
The protocol page opened on 2026-09-18 states subcutaneous figures of 50 to 100 mg per injection two to three times a week, works its examples at 100 mg/mL (500 mg in 5 mL, 750 mg in 7.5 mL, 1,000 mg in 10 mL) and 200 mg/mL (500 mg in 2.5 mL, 1,000 mg in 5 mL), and states that direct human evidence for subcutaneous schedules is limited (Peptide Dosing Protocols, NAD+ guide, last updated August 2026).
The same protocol page states that unbuffered NAD+ reconstitutes acidic, at about pH 3 to 4, and is more often associated with stinging at the injection site than buffered presentations that ship with a pH stabiliser.
At 100 mg/mL a 100 mg amount is 1 mL, the full barrel of a U-100 syringe; at 50 mg/mL it is 2 mL, more than one insulin syringe holds. Scaling the water in proportion to the vial leaves the concentration unchanged; only less water per milligram makes a given amount occupy a smaller volume.

Frequently asked questions

How much water goes into a 500 mg vial of NAD+?

No label sets it. The protocol page opened on 2026-09-18 works a 500 mg vial at 5 mL of bacteriostatic water, which is 100 mg/mL, and at 2.5 mL, which is 200 mg/mL. The US registration NCT06919328 dissolves 100 mg in 2 mL, which is 50 mg/mL. The calculator's standard 1, 2 and 3 mL columns give 500, 250 and 167 mg/mL for a 500 mg vial, concentrations no source opened for this page uses; the sources all add more water per milligram than a peptide vial gets.

How many units is 100 mg of NAD+ on an insulin syringe?

At 100 mg/mL, 100 units, which is the entire 1 mL barrel. At 200 mg/mL, 50 units. At 50 mg/mL, 200 units, which is 2 mL and does not fit a 1 mL insulin syringe at all; the registration that uses that concentration describes a 2 mL injection. The figure only means something next to the water volume that produced it, and for NAD+ the volumes are large enough that the syringe size itself becomes a constraint.

Why is NAD+ reconstitution different from a peptide's?

Because the vial is 10 to 200 times larger in mass. A 10 mg peptide vial in 2 mL is 5 mg/mL and one U-100 unit carries 50 mcg; a 500 mg NAD+ vial in the same 2 mL would be 250 mg/mL and one unit would carry 2,500 mcg. The amounts the sources state are also milligrams, so the draws are whole millilitres rather than a few units. Nothing about the formula changes; every number in it is an order of magnitude bigger.

Is there a labelled or trial dose for NAD+ injection?

There is no label: DailyMed holds no NAD+ injection product. The human injection record spans a wide range. A randomized trial in heart failure gave 10 mg intravenously a day; a US registration gives 100 mg in 2 mL by three injection routes; another gives 500 mg by infusion in 500 mL of saline; and the one pharmacokinetic study infused about 716 mg over six hours. No dose-finding study connects those figures, and none of them is a subcutaneous wellness figure.

Is NAD+ a peptide?

No. It is nicotinamide adenine dinucleotide, a coenzyme made of two nucleotides joined through a phosphate bridge, with no amino acids in it (PubChem CID 5892). It is sold through the same vendors and prepared with the same bacteriostatic water and insulin syringes as research peptides, which is why it has a calculator and a page here, but it belongs to a different chemical class and its vial sizes reflect that.

What happens to NAD+ after it is infused?

In the only published human study of the question, Grant and colleagues infused 3 µmol per minute for six hours and sampled plasma and urine throughout. Plasma NAD+ and its metabolites did not change for the first two hours; the authors report that at that rate NAD+ is rapidly and completely removed from the plasma, with a metabolite pattern consistent with enzymes that break it down outside cells. By six hours, urinary methylnicotinamide and NAD+ itself had risen. The study measured no clinical outcome.