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CJC-1295 Reconstitution: Water per Vial, mg/mL and Units

Water volumes for 5 and 10 mg CJC-1295 vials, the mg/mL each gives, and U-100 units for the trial and circulating figures, DAC and no-DAC alike.

Medibact Guides · Published 2026-09-18

Illustration: Two clear glass vials, one with pale blue liquid, one empty, and an insulin syringe on a white surface.
Illustration

This page does the arithmetic for CJC-1295 vials: water volume per vial size, the concentration it produces, and what the trial figures and the circulating figures come to in U-100 syringe units. Every figure carries its source and the date it was read. Nothing here is a recommendation.

CJC-1295 is a synthetic analogue of growth-hormone-releasing hormone, specifically of its first 29 amino acids, GHRH(1-29). The name covers two molecules. CJC-1295 with DAC carries a "drug affinity complex", a chemical group that binds the molecule to albumin in the blood after injection and is the reason its half-life in the 2006 human trials was measured in days. What the research market sells as "CJC-1295 no DAC" is the same modified GHRH(1-29) without that complex, also sold as Mod GRF 1-29, with a half-life measured in minutes. The distinction matters for what a vial contains and for which reported figure applies to it; it does not change the reconstitution arithmetic, which depends only on the milligrams in the vial and the millilitres of water added. The CJC-1295 calculator works any combination, and Medibact's CJC-1295 guide covers the compound itself.

What the sources hold

No label: a DailyMed search on 2026-09-18 returned zero records for CJC-1295, so no regulator has reviewed a dose or a storage condition.

One registration: ClinicalTrials.gov held a single study naming CJC-1295 as an intervention on 2026-09-18, NCT00267527, a ConjuChem phase 2 study of 12 weeks' treatment in HIV-infected patients with visceral obesity, planned for 120 participants, started December 2005, listed as terminated with no results posted and no reason recorded.

Two human trials, both of the DAC form. Teichman et al. (JCEM 2006, PMID 16352683) ran two randomised placebo-controlled studies of 28 and 49 days in healthy adults aged 21 to 61: four ascending single subcutaneous doses in the first, two or three weekly or biweekly doses in the second. GH rose 2- to 10-fold for 6 days or more and IGF-I 1.5- to 3-fold for 9 to 11 days after one injection; the estimated half-life was 5.8 to 8.1 days; after multiple doses IGF-I stayed above baseline for up to 28 days; the authors describe the drug as relatively well tolerated, "particularly at doses of 30 or 60 microg/kg". Ionescu and Frohman (JCEM 2006, PMID 17018654) sampled blood every 20 minutes overnight in healthy men aged 20 to 40 before and one week after a single injection of 60 or 90 mcg/kg: pulsatility was preserved, trough GH rose 7.5-fold, mean GH 46% and IGF-I 45%, with no difference between the two doses. PubMed holds 33 title-and-abstract records for CJC-1295 on 2026-09-18; the trial filter returns those two and nothing else. Most of the rest is anti-doping analytical chemistry.

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The trial doses, scaled to body weight

The trials stated doses per kilogram. Scaling them is arithmetic, and it shows that the trial quantities were milligrams, not the microgram figures that circulate for the no-DAC form.

Trial dose (DAC form) 60 kg 70 kg 80 kg 90 kg
30 mcg/kg 1.8 mg 2.1 mg 2.4 mg 2.7 mg
60 mcg/kg 3.6 mg 4.2 mg 4.8 mg 5.4 mg
90 mcg/kg 5.4 mg 6.3 mg 7.2 mg 8.1 mg

Water per vial and the resulting concentration

The vial sizes the site's calculator carries are 5 mg and 10 mg, the common research presentations. Concentration is the vial amount divided by the water volume.

Vial 1 mL of water 2 mL of water 3 mL of water
5 mg 5 mg/mL 2.5 mg/mL 1.67 mg/mL
10 mg 10 mg/mL 5 mg/mL 3.33 mg/mL

Units on a U-100 syringe for the reported amounts

Units = quantity in mg divided by mg/mL, times 100. The microgram rows are the figures protocol and vendor pages state for the no-DAC form (100 to 300 mcg per injection, read 2026-09-18); the milligram rows are those pages' DAC figures (1 to 2 mg a week) and the lowest trial dose scaled to 80 kg (2.4 mg).

Quantity 1.67 mg/mL 2.5 mg/mL 3.33 mg/mL 5 mg/mL 10 mg/mL
100 mcg 6 units 4 units 3 units 2 units 1 unit
200 mcg 12 units 8 units 6 units 4 units 2 units
300 mcg 18 units 12 units 9 units 6 units 3 units
1 mg 60 units 40 units 30 units 20 units 10 units
2 mg 120 units (two syringes) 80 units 60 units 40 units 20 units
2.4 mg 144 units (two syringes) 96 units 72 units 48 units 24 units

The table shows why the two forms are usually worked at different concentrations. Microgram quantities occupy one or two units at 10 mg/mL, below what a 1 mL syringe's 2-unit markings resolve, and become readable draws only with more water. Milligram quantities exceed one syringe at the most dilute mixes. The insulin syringe units page covers the scales and barrel sizes.

DAC and no-DAC: same arithmetic, different figures

Because the two molecules share a name, a unit figure quoted for one is routinely applied to the other. The tables above make the consequence visible: a 2 mg weekly quantity for the DAC form is 40 units at 5 mg/mL, while a 100 mcg quantity for the no-DAC form at the same concentration is 2 units, a twenty-fold difference in the draw from the same vial and water. Which molecule a vial holds is stated on its own label. The site keeps a separate Mod GRF 1-29 calculator for the no-DAC form, a CJC-1295 with ipamorelin calculator for the blended vial, and a combination article on the pairing.

The mixing steps, as labels describe them

No CJC-1295 label exists, so the steps come from labelled peptide products prepared the same way. The EGRIFTA WR label (tesamorelin, revised 3/2025) directs that the diluent be added to the vial and the vial moved in a circle to mix, and states: do not shake. The EGRIFTA SV label (revised 2/2024) states that the vial is mixed by rolling it gently between the hands for 30 seconds, and again: do not shake (DailyMed, read 2026-09-18). The general procedure for research vials is on Medibact's page on how to reconstitute peptides; this page does not repeat it.

Storage after mixing

No product label states a storage condition for reconstituted CJC-1295; the EGRIFTA WR label's figure of 20 to 25 degrees Celsius for up to 7 days applies to that product alone, and Medibact's article on how to store peptides covers storage of reconstituted research vials.

What the figures omit

The trial figures omit everything a circulating figure claims to know. They were single or few doses of the DAC form in healthy adults, measured for GH and IGF-I over weeks, not months of use, not muscle or fat, and not the no-DAC form at all. The circulating figures omit their origin: the 1 to 2 mg weekly DAC figure sits below the lowest trial dose for most adults, and the 100 to 300 mcg no-DAC figure has no human study behind it. Route (subcutaneous in the trials), frequency, duration and population are therefore reported facts about two 2006 papers, not properties of the compound. The site's CJC-1295 dosage page sets every figure against its source.

The CJC-1295 calculator returns concentration and units for any vial and water volume. The reconstitution hub indexes every compound page and the chart. The muscle-growth stack page and the sermorelin and ipamorelin page cover the combinations this compound is sold in, and the blend calculator does the arithmetic for two vials drawn together.

This page does not recommend a dose, a water volume, a form or a schedule. It reports what two trials, one registry and the circulating pages state, and it does the arithmetic on the vial sizes sold.

Sources and dates

Opened 2026-09-18: the DailyMed SPL search API for CJC-1295 (zero records); the ClinicalTrials.gov v2 API for CJC-1295 as an intervention (one record) and the NCT00267527 record; PubMed through the NCBI E-utilities for "CJC-1295"[tiab] (33 records) and the trial-type filter (2 records), with the abstracts of PMID 16352683 and 17018654 retrieved in full; the EGRIFTA WR and EGRIFTA SV labels on DailyMed (setids 839334d3-8c1d-4c26-9036-2ab524a6ea75 and 3d783378-b02d-4f19-99dd-0fc91a042224) for the mixing and storage wording; protocol and vendor pages carrying DAC and no-DAC figures, read the same day. Corrections go to the contact page.

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Frequently asked questions

How much water goes into a 5 mg CJC-1295 vial?

No label states a volume. The concentration is the vial amount divided by the water added: 1 mL gives 5 mg/mL, 2 mL gives 2.5 mg/mL and 3 mL gives 1.67 mg/mL. The water changes how many syringe units a given quantity occupies, not the quantity itself: 100 mcg is 2 units at 5 mg/mL, 4 units at 2.5 mg/mL and 6 units at 1.67 mg/mL. Small quantities measure more easily at lower concentrations; milligram quantities fit in one syringe only at higher ones. The site's CJC-1295 calculator works any combination.

Is reconstitution different for CJC-1295 with DAC and without DAC?

The arithmetic is identical: concentration depends only on the milligrams in the vial and the millilitres of water added. What differs is the figure being drawn. The DAC form is the molecule in the 2006 human trials and on circulating pages carries weekly milligram figures; the no-DAC form, sold as Mod GRF 1-29, carries daily microgram figures on those same pages and has no human trial. Which molecule the vial holds is stated on its own label, and the site keeps a separate Mod GRF 1-29 calculator for that reason.

How many units is 100 mcg of CJC-1295 on an insulin syringe?

At 5 mg/mL (a 10 mg vial in 2 mL, or a 5 mg vial in 1 mL) 100 mcg is 0.02 mL, which is 2 units on a U-100 syringe. At 2.5 mg/mL it is 4 units, at 10 mg/mL 1 unit, at 1.67 mg/mL 6 units. Two units sits at the limit of what a 1 mL syringe's 2-unit markings can show, which is why microgram quantities are usually worked at lower concentrations, where the same quantity occupies more units.

What dose of CJC-1295 was used in human trials?

Both trials used the DAC form in healthy adults. Teichman et al. (2006) gave four ascending single subcutaneous doses and then two or three weekly or biweekly doses, and reported the drug as relatively well tolerated particularly at 30 or 60 mcg/kg; Ionescu and Frohman (2006) gave a single dose of 60 or 90 mcg/kg. For a 70 kg adult those per-kilogram figures are 2.1, 4.2 and 6.3 mg. No human trial of the no-DAC form exists, and no trial measured muscle or body composition in healthy people.

Can CJC-1295 and ipamorelin be mixed in one vial?

Pre-mixed blend vials are sold, and the number on a blend vial is normally the combined mass of both peptides, often split 5 mg and 5 mg in a 10 mg vial, though the split is set by whoever filled it. A blend fixes the ratio of the two peptides at fill time: drawing more or less scales both together. The site's blend calculator handles the per-component arithmetic, and its combination article on CJC-1295 with ipamorelin covers the pairing itself.

How long does reconstituted CJC-1295 last?

No label exists to state a duration for this compound, so any figure offered for it is a convention rather than a labelled fact. Storage of reconstituted vials is Medibact's topic, and its storage article is linked from this page rather than repeated here.