Ipamorelin Reconstitution: Water per Vial, mg/mL and Units

How 5 mg and 10 mg ipamorelin vials convert to mg/mL and U-100 units for the 100 to 300 mcg figures protocol pages state, beside the intravenous trial doses.

Medibact Guides · Published 2026-09-18

Illustration: Two clear glass vials on a white bench, one with blue liquid, one empty. — Illustration

Ipamorelin is a pentapeptide, five amino acids, developed by Novo Nordisk in the 1990s as a selective growth-hormone secretagogue and described in the European Journal of Endocrinology in 1998. On the research market it is sold as a lyophilized powder in 5 mg and 10 mg vials, the sizes the ipamorelin calculator is built around, both on its own and blended with CJC-1295. This page covers the single-compound vial: the water volumes the sources state, the concentration each produces, and what the microgram figures those sources quote come to on a U-100 syringe. It reports and does not recommend. The figures and their provenance are on the ipamorelin dosage page; the blended vial has its own reconstitution page; every compound's arithmetic is indexed on the reconstitution hub.

What the sources state

Source	What it states	Kind of source	Opened
DailyMed search, "ipamorelin"	Zero labels	Regulator database	2026-09-18
ClinicalTrials.gov, ipamorelin as intervention	Three records: NCT00672074 (Phase 2, 117 enrolled, intravenous, completed) and NCT01280344 (Phase 2, 320 enrolled, 0.03 and 0.06 mg/kg intravenously twice or three times daily, completed, no results posted), plus one observational study	Trial registry	2026-09-18
Raun et al., Eur J Endocrinol 1998	Ipamorelin is the pentapeptide Aib-His-D-2-Nal-D-Phe-Lys-NH2; releases growth hormone in rats and pigs without raising ACTH or cortisol	Peer-reviewed pharmacology paper	2026-09-18
Gobburu et al., Pharm Res 1999	Infusions of 4.21, 14.02, 42.13, 84.27 and 140.45 nmol/kg over 15 minutes, eight healthy men per level; half-life 2 hours; a single GH pulse at every dose	Peer-reviewed pharmacokinetic study	2026-09-18
Beck et al., Int J Colorectal Dis 2014	0.03 mg/kg intravenously twice daily, postoperative day 1 to 7; time to first tolerated meal 25.3 vs 32.6 hours, p = 0.15	Peer-reviewed randomized trial	2026-09-18
Peptide Dosing Protocols, ipamorelin guide	"100 to 300 mcg per shot, one to three times per day"; 2 mg in 1.0 mL (200 mcg is 10 units), 5 mg in 2.0 mL (8 units), 10 mg in 3.0 mL (6 units); "This schedule is community-derived, not from clinical trials. No human trial has tested ipamorelin for GH optimization, body composition, or anti-aging endpoints"	Protocol site, last reviewed August 2026	2026-09-18

The human record and the research-vial convention do not touch. The trials are intravenous, per kilogram, in a hospital; the pages describe fixed microgram amounts under the skin. The protocol page says so in its own words.

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Water per vial and the concentration it gives

The calculator works both vials at 1, 2 and 3 mL; the protocol page's 5 mg in 2 mL and 10 mg in 3 mL are two of those rows, and its 2 mg vial is added for completeness. One U-100 unit is a hundredth of a millilitre (MedlinePlus, "Giving an insulin injection", reviewed 2024-07-21), so the micrograms in one unit are the concentration in mcg/mL divided by 100.

Vial	Water added	Concentration	Micrograms per unit
2 mg	1 mL	2 mg/mL	20 mcg
5 mg	1 mL	5 mg/mL	50 mcg
5 mg	2 mL	2.5 mg/mL	25 mcg
5 mg	3 mL	1.67 mg/mL	16.7 mcg
10 mg	1 mL	10 mg/mL	100 mcg
10 mg	2 mL	5 mg/mL	50 mcg
10 mg	3 mL	3.33 mg/mL	33.3 mcg

A 10 mg vial in 2 mL and a 5 mg vial in 1 mL are the same solution; the larger vial simply holds twice as many draws of it.

The stated microgram amounts in syringe units

The protocol page's range is 100 to 300 mcg per injection. The table converts the two ends and the middle at every configuration above.

Vial and water	100 mcg	200 mcg	300 mcg
2 mg in 1 mL	5 units	10 units	15 units
5 mg in 1 mL	2 units	4 units	6 units
5 mg in 2 mL	4 units	8 units	12 units
5 mg in 3 mL	6 units	12 units	18 units
10 mg in 1 mL	1 unit	2 units	3 units
10 mg in 2 mL	2 units	4 units	6 units
10 mg in 3 mL	3 units	6 units	9 units

The 10 mg in 1 mL row explains the protocol page's choice of 3 mL for the larger vial: a 1-unit draw cannot be read to better than a half unit, which at that concentration is half the amount. The 2 mg vial's 1 mL is the same idea from the other end, a small vial kept concentrated enough that a 200 mcg amount is still a 10-unit reading. Both are statements about the graduations on a syringe, not about the amounts.

The trial dose beside the conventions

For scale only: the trial dose of 0.03 mg/kg is 2.25 mg for a 75 kg adult, given intravenously twice a day in surgical patients. At 2.5 mg/mL that would be 90 units, nearly a full barrel, against the 4 to 12 units of the subcutaneous conventions. The comparison shows how far apart the two sets of figures are; it is not a conversion any source makes, and the route alone would make it meaningless. The pharmacokinetic study adds the other relevant fact: a half-life of 2 hours after infusion, and a single growth-hormone pulse at every dose level tested.

The mixing steps, as a label describes them

Ipamorelin has no label. The labelled procedure for a lyophilized peptide supplied with bacteriostatic water is in the EGRIFTA WR (tesamorelin) prescribing information revised in March 2025: only the supplied Bacteriostatic Water for Injection is used; the stated volume is added to the vial; the vial is moved in a circle to mix the powder and liquid and is not shaken; the solution is inspected and not used if particles appear or it is cloudy or coloured. The same label states its concentration as 8 mg/mL for 11.6 mg in 1.3 mL, not the 8.9 mg/mL of simple division, because the dissolved powder adds to the final volume, a displacement every calculator, including this site's, leaves out. The general procedure for any lyophilized vial is on Medibact's how to reconstitute peptides page and is not repeated here.

Storage after mixing

There is no ipamorelin label to quote on the solution's storage; what labels and manufacturers state about reconstituted peptides is on Medibact's how to store peptides page.

What this page does not do

It does not state an amount of ipamorelin to use, a time of day, a frequency or a cycle. The 100 to 300 mcg figures are reported because a protocol page states them, with that page's own statement that they are community-derived and untested in any human trial; the trial figures are reported because they are the only human doses that exist, and they are intravenous and per kilogram. The arithmetic converts stated inputs exactly and says nothing about whether any input is appropriate. Where the peptide is combined with CJC-1295, the blend calculator handles the two masses separately. Medibact's Ipamorelin guide covers the compound; Peptifact's CJC-1295 and ipamorelin dosage page sets the trial doses against the clinic doses. Decisions about administering anything to a person belong with a clinician.

Sources and dates

Opened 2026-09-18: the DailyMed SPL search API for "ipamorelin" (zero records); the ClinicalTrials.gov v2 API for ipamorelin as an intervention (three records) and the study records NCT00672074 and NCT01280344; PubMed through the NCBI E-utilities for the abstracts of PMID 9849822 (Raun et al. 1998), PMID 10496658 (Gobburu et al. 1999) and PMID 25331030 (Beck et al. 2014) and the counts for ipamorelin[tiab] (50) and ipamorelin[tiab] AND humans[mh] (24); the Peptide Dosing Protocols ipamorelin guide (last reviewed August 2026); the EGRIFTA WR prescribing information (revised 3/2025, sections 2.2 and 11); MedlinePlus, "Giving an insulin injection" (reviewed 2024-07-21). Corrections go to the contact page.

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Medibact Guides facts

Ipamorelin is a five-amino-acid growth-hormone secretagogue, Aib-His-D-2-Nal-D-Phe-Lys-NH2, first described by Novo Nordisk in 1998 (Raun et al., European Journal of Endocrinology). Research vials are 5 mg and 10 mg, the sizes the calculator carries; a protocol page also works a 2 mg vial.
No approved product contains ipamorelin: a DailyMed search on 2026-09-18 returned zero labels, so no label states a diluent volume, a concentration or a dose for it.
Ipamorelin's human doses are intravenous and weight-based. Gobburu et al. (Pharmaceutical Research 1999) infused 4.21 to 140.45 nmol/kg over 15 minutes in eight healthy men per level and found a half-life of 2 hours; Beck et al. (International Journal of Colorectal Disease 2014) gave 0.03 mg/kg intravenously twice daily for up to 7 days to bowel-resection patients, 117 enrolled, with no significant difference from placebo in time to a tolerated meal.
ClinicalTrials.gov held three ipamorelin records on 2026-09-18: the two completed Phase 2 trials in bowel-surgery patients, NCT00672074 (117 enrolled) and NCT01280344 (320 enrolled, arms of 0.03 and 0.06 mg/kg intravenously twice or three times daily, no results posted), and one observational study. No registered trial has given ipamorelin subcutaneously for any purpose.
The figures protocol and clinic pages state are 100 to 300 mcg per subcutaneous injection, one to three times a day; the protocol page opened on 2026-09-18 states that its schedule is community-derived and not from clinical trials, and that no human trial has tested ipamorelin for growth-hormone optimisation, body composition or anti-aging endpoints (Peptide Dosing Protocols, last reviewed August 2026).
That page works three configurations: a 2 mg vial in 1 mL (2,000 mcg/mL, 200 mcg is 10 units), a 5 mg vial in 2 mL (2,500 mcg/mL, 200 mcg is 8 units) and a 10 mg vial in 3 mL (3,333 mcg/mL, 200 mcg is 6 units).
At the calculator's water volumes a 5 mg vial gives 5, 2.5 and 1.67 mg/mL in 1, 2 and 3 mL, so 100 mcg is 2, 4 and 6 units on a U-100 syringe and 300 mcg is 6, 12 and 18; a 10 mg vial gives 10, 5 and 3.33 mg/mL, so 100 mcg is 1, 2 and 3 units and 300 mcg is 3, 6 and 9.
The trial dose of 0.03 mg/kg is 2.25 mg for a 75 kg adult, an intravenous figure roughly eight to twenty-three times the subcutaneous conventions of 100 to 300 mcg; at 2.5 mg/mL it would be 90 units, a conversion shown only to make the scale visible, since the route is different and no source applies it to a vial.
PubMed held 50 records with ipamorelin in the title or abstract on 2026-09-18, 24 of them tagged for humans.

Frequently asked questions

How much water goes into a 5 mg vial of ipamorelin?

No label sets it. The protocol page opened on 2026-09-18 works a 5 mg vial at 2 mL of bacteriostatic water, 2,500 mcg/mL, and a 10 mg vial at 3 mL, 3,333 mcg/mL. The calculator's columns give a 5 mg vial 5, 2.5 and 1.67 mg/mL at 1, 2 and 3 mL, and a 10 mg vial 10, 5 and 3.33 mg/mL. The volume sets how many units each microgram amount occupies; the 5 mg in the vial is the same whichever volume is chosen.

How many units is 200 mcg of ipamorelin on an insulin syringe?

Eight units at 2.5 mg/mL, the protocol page's 5 mg in 2 mL. Four units at 5 mg/mL (5 mg in 1 mL, or 10 mg in 2 mL). Six units at 3.33 mg/mL (10 mg in 3 mL). Twelve units at 1.67 mg/mL (5 mg in 3 mL). Two units at 10 mg/mL (10 mg in 1 mL). Ten units at 2 mg/mL, the page's 2 mg vial in 1 mL. A 100 mcg amount is half of each and 300 mcg is one and a half times each.

What doses did the human trials of ipamorelin use?

Intravenous ones, per kilogram, in a pharmacology study and in surgical patients. Gobburu and colleagues infused 4.21 to 140.45 nmol/kg over 15 minutes in healthy men and measured a 2-hour half-life; Beck and colleagues gave 0.03 mg/kg twice daily for up to seven days after bowel resection, and a second registered trial added a 0.06 mg/kg arm. For a 75 kg adult, 0.03 mg/kg is 2.25 mg per infusion, far above the microgram figures pages state for a research vial, by a different route, for a different purpose.

Is ipamorelin approved anywhere?

No. DailyMed holds no ipamorelin label. The two completed Phase 2 trials were of an intravenous product for postoperative ileus, and the published one found no significant difference from placebo on its main endpoint; the larger one has posted no results. There is no approved product, no labelled dose and no subcutaneous trial, which is what the protocol page means when it calls its own schedule community-derived.

How is ipamorelin reconstituted?

As labels describe for any lyophilized peptide supplied with bacteriostatic water, since it has no label of its own. The EGRIFTA WR label, revised March 2025, has the stated volume of diluent added to the vial, the vial moved in a circle to mix rather than shaken, and the solution inspected and used only if clear and free of particles. The general procedure, step by step, is on Medibact's reconstitution page; this page carries the arithmetic for the 5 mg and 10 mg vials.

What changes when ipamorelin is in a blend with CJC-1295?

The mass on the vial becomes the combined mass of both peptides, and every per-peptide figure depends on the split, which is set at fill and not standardised. A 10 mg blend at 5 mg plus 5 mg in 2 mL gives 2.5 mg/mL of each, so 100 mcg of each is 4 units, and any draw delivers both peptides in the vial's ratio. The blend's own page on this site carries that arithmetic, and the blend calculator handles uneven splits and two-vial preparations.