CJC-1295 Ipamorelin Reconstitution: Blend Water and Units

How a 5 mg or 10 mg CJC-1295 and ipamorelin blend vial converts to mg/mL of each peptide and U-100 units, with the label-mass arithmetic explained.

Medibact Guides · Published 2026-09-18

Illustration: A partially filled vial and an empty lyophilized vial with an insulin syringe on a white surface. — Illustration

CJC-1295 is a 29-amino-acid analogue of growth-hormone-releasing hormone, and ipamorelin is a five-amino-acid growth-hormone secretagogue first described by Novo Nordisk in 1998. On the research market the two are most often supplied together in one lyophilized vial, labelled 5 mg or 10 mg, and it is that blended vial the blend preset of the reconstitution calculator is built around. This page reports the water volumes the sources state, the concentration of the blend and of each half at each volume, and what the microgram figures those sources quote come to on a U-100 syringe. It does not recommend anything. The figures and their provenance are on the CJC-1295 and ipamorelin dosage page; every compound's arithmetic is indexed on the reconstitution hub.

The number on the label is the combined mass

Everything about a blend vial follows from one fact that pages routinely get wrong: the milligram figure is the total peptide in the vial, not the amount of each. A 10 mg blend usually contains 10 mg of peptide altogether, a common fill being 5 mg of CJC-1295 and 5 mg of ipamorelin. Some vials are filled at other ratios; some list only the total; none of it is standardised.

The arithmetic is therefore done twice. The blend concentration is the total mass divided by the water: 10 mg in 2 mL is 5 mg/mL. Each peptide's concentration is its own mass divided by the same water: 5 mg in 2 mL is 2.5 mg/mL. Dividing the blend concentration by two gives the right answer only at an exactly even split, which is why the per-peptide figures on this page carry the words "at a one-to-one fill".

Bacteriostatic Water

USP-grade, 0.9% benzyl alcohol, 30 mL multi-dose vial. Research use only.

$30.99

Buy at Medibact

What the sources state

Source	What it states	Kind of source	Opened
DailyMed searches, "CJC-1295" and "ipamorelin"	Zero labels for either	Regulator database	2026-09-18
ClinicalTrials.gov	CJC-1295: one record, NCT00267527, Phase 2 in HIV-associated visceral obesity, terminated. Ipamorelin: NCT00672074 (117 enrolled) and NCT01280344 (320, arms of 0.03 and 0.06 mg/kg intravenously twice or three times daily), both completed Phase 2 trials in bowel-surgery patients, no results posted	Trial registry	2026-09-18
Teichman et al., JCEM 2006	CJC-1295 with DAC, subcutaneous, healthy adults, two placebo-controlled trials; "well tolerated, particularly at doses of 30 or 60 microg/kg"; half-life 5.8 to 8.1 days	Peer-reviewed trial	2026-09-18
Gobburu et al., Pharmaceutical Research 1999	Ipamorelin infused at 4.21 to 140.45 nmol/kg over 15 minutes, eight men per level; half-life 2 hours	Peer-reviewed pharmacology study	2026-09-18
Beck et al., Int J Colorectal Dis 2014	Ipamorelin 0.03 mg/kg intravenously twice daily, days 1 to 7 after bowel resection; time to first tolerated meal 25.3 vs 32.6 hours, not significant	Peer-reviewed trial	2026-09-18
Beverly Hills Rejuvenation Center guide	5 mg plus 5 mg vial in 2 mL: "25 mcg per unit of each peptide"; 100 mcg of each once daily at bedtime five days a week, titrated to 200 to 300 mcg of each	Clinic guide, dated 2026-07-24, updated 2026-09-11	2026-09-18
Peptide Dosing Protocols, ipamorelin guide	"100 to 300 mcg per shot, one to three times per day"; "community-derived, not from clinical trials"	Protocol site, last reviewed August 2026	2026-09-18

The trial doses and the blend figures do not connect. The 2006 trial is of the DAC form, per kilogram, weekly; the blend sold with ipamorelin most often contains the no-DAC form, also sold as Mod GRF 1-29, at a fixed microgram figure nightly. The ipamorelin trials are intravenous and per kilogram in a hospital. Converting either into a subcutaneous blend figure would require a study that does not exist.

Water per vial and the concentration of each half

The table assumes a one-to-one fill. U-100 means 100 units in 1 mL (MedlinePlus, reviewed 2024-07-21), so the micrograms in one unit are the concentration in mcg/mL divided by 100.

Vial (combined)	Water	Blend	Each peptide	Micrograms of each per unit
5 mg (2.5 + 2.5)	1 mL	5 mg/mL	2.5 mg/mL	25 mcg
5 mg	2 mL	2.5 mg/mL	1.25 mg/mL	12.5 mcg
5 mg	3 mL	1.67 mg/mL	0.83 mg/mL	8.3 mcg
10 mg (5 + 5)	1 mL	10 mg/mL	5 mg/mL	50 mcg
10 mg	2 mL	5 mg/mL	2.5 mg/mL	25 mcg
10 mg	3 mL	3.33 mg/mL	1.67 mg/mL	16.7 mcg

The stated microgram amounts in syringe units

The clinic and protocol pages quote 100, 200 and 300 mcg of each peptide per injection. These are the draws that deliver those amounts of each, at a one-to-one fill; each draw also delivers the same number of micrograms of the other peptide, because both are in the same solution.

Vial and water	100 mcg of each	200 mcg of each	300 mcg of each
5 mg in 1 mL, or 10 mg in 2 mL	4 units	8 units	12 units
5 mg in 2 mL	8 units	16 units	24 units
5 mg in 3 mL	12 units	24 units	36 units
10 mg in 1 mL	2 units	4 units	6 units
10 mg in 3 mL	6 units	12 units	18 units

The 10 mg in 1 mL row shows why the sources chose 2 mL: a 2-unit draw sits at the limit of what the graduations on a U-100 syringe can resolve, and a half-unit error is a quarter of the amount. The 5 mg in 3 mL row shows the opposite cost, a draw large enough to make a 10 mg vial's worth of injections into a larger number of draws than the vial's fill suggests. Neither row is wrong; each is the same vial at a different volume.

One vial locks the ratio

Because both peptides dissolve in the same water, every draw carries them in the proportion the vial was filled at. Drawing 12 units instead of 4 triples both; nothing done at the syringe can raise one without the other. That is the real difference between a blended vial and two separate vials, and it is a property of the arithmetic rather than a recommendation about which to use. Where the two are in separate vials, or a blend is filled at an uneven ratio, the blend calculator takes each mass separately, and the single-compound presets for CJC-1295 and ipamorelin handle each vial on its own; the ipamorelin reconstitution page carries that compound's arithmetic alone.

The mixing steps, as a GHRH-analogue label describes them

No label exists for either peptide or the blend. The labelled procedure for a lyophilized GHRH analogue supplied with bacteriostatic water is in the EGRIFTA WR (tesamorelin) prescribing information, revised March 2025: only the supplied Bacteriostatic Water for Injection is used; 1.3 mL is added to the vial; the vial is moved in a circle to mix the powder and liquid and is not shaken; the solution is inspected and not used if particles appear or it is cloudy or coloured. That label also states its concentration as 8 mg/mL for 11.6 mg in 1.3 mL, lower than the 8.9 mg/mL of simple division, because the dissolved powder adds to the final volume; the calculator's figures, like all such figures, ignore that small displacement. The general procedure is on Medibact's how to reconstitute peptides page.

Storage after mixing

There is no label for either peptide to quote on storage of the solution; what labels and manufacturers state for reconstituted peptides is on Medibact's how to store peptides page.

What this page does not do

It does not state an amount of either peptide to use, a time of day, a frequency or a cycle. The 100 to 300 mcg figures are reported because clinic and protocol pages state them, with the protocol page's own statement that they are community-derived; the trial figures are reported because they are the only human doses that exist, and they are of different molecules by different routes. The arithmetic is exact for its inputs and silent on whether any input is appropriate. This site's CJC-1295 and ipamorelin article covers the combination itself; the compounds are covered in Medibact's CJC-1295 guide and Medibact's Ipamorelin guide. Decisions about administering anything to a person belong with a clinician.

Sources and dates

Opened 2026-09-18: the DailyMed SPL search API for "CJC-1295" and "ipamorelin" (zero records each); the ClinicalTrials.gov v2 API for both as intervention terms and the study records NCT00267527, NCT00672074 and NCT01280344; PubMed through the NCBI E-utilities for the abstracts of PMID 16352683 (Teichman et al. 2006), PMID 10496658 (Gobburu et al. 1999), PMID 25331030 (Beck et al. 2014) and PMID 9849822 (Raun et al. 1998, the pentapeptide's first description); the Beverly Hills Rejuvenation Center CJC-1295/ipamorelin guide (2026-07-24, updated 2026-09-11); the Peptide Dosing Protocols ipamorelin guide (last reviewed August 2026); the EGRIFTA WR prescribing information (revised 3/2025, sections 2.2 and 11); MedlinePlus, "Giving an insulin injection" (reviewed 2024-07-21). Corrections go to the contact page.

Bacteriostatic Water

USP-grade, 0.9% benzyl alcohol, 30 mL multi-dose vial. Research use only.

$30.99

Buy at Medibact

Medibact Guides facts

The milligram figure on a CJC-1295 and ipamorelin blend vial is the combined mass of both peptides: a vial labelled 10 mg normally holds 10 mg of peptide in total, often 5 mg of each, and the split is set by whoever filled it rather than by any standard.
At a 5 mg plus 5 mg fill in 2 mL of bacteriostatic water, the blend is 5 mg/mL and each peptide is 2.5 mg/mL, so one U-100 unit carries 25 mcg of each; 100 mcg of each is 4 units, 200 mcg is 8 and 300 mcg is 12 (the same arithmetic a clinic guide published on 2026-07-24 and updated 2026-09-11 states).
Neither peptide has an approved product: DailyMed searches for CJC-1295 and for ipamorelin on 2026-09-18 each returned zero labels, so no label states a diluent volume, a concentration or a dose for either, or for the blend.
No human trial has given the two peptides together. ClinicalTrials.gov on 2026-09-18 held one CJC-1295 record (NCT00267527, a Phase 2 study in HIV-associated visceral obesity, terminated) and two completed ipamorelin Phase 2 trials (NCT00672074 and NCT01280344), both of intravenous ipamorelin after bowel surgery.
The CJC-1295 that was studied in people is the DAC form: Teichman et al. (JCEM 2006) gave it subcutaneously to healthy adults in two placebo-controlled trials and report it as well tolerated particularly at 30 or 60 mcg/kg, with a half-life of 5.8 to 8.1 days. Blends sold with ipamorelin most often contain the no-DAC form, also sold as Mod GRF 1-29, which is a different molecule.
Ipamorelin's human doses are intravenous and weight-based: 4.21 to 140.45 nmol/kg infused over 15 minutes in healthy men (Gobburu et al., Pharmaceutical Research 1999, half-life 2 hours) and 0.03 mg/kg twice daily for up to 7 days in 117 bowel-resection patients, with no significant difference from placebo on the main endpoint (Beck et al., International Journal of Colorectal Disease 2014).
The figures protocol and clinic pages state for the blend are 100 mcg of each peptide per subcutaneous injection, rising to 200 to 300 mcg of each, once to three times a day; the protocol page states its schedule is community-derived and not from clinical trials (Peptide Dosing Protocols, ipamorelin guide, last reviewed August 2026; Beverly Hills Rejuvenation Center guide, 2026-07-24; both opened 2026-09-18).
A single blended vial locks the ratio between the two peptides at fill time: a larger or smaller draw scales both by the same factor. Only two separate vials allow the amounts to move independently, which is what the blend calculator on this site is for.

Frequently asked questions

How much water goes into a 10 mg CJC-1295 ipamorelin vial?

No label sets it, so the volume is a convention. The clinic guide opened on 2026-09-18 describes 2 mL of bacteriostatic water in a 5 mg plus 5 mg vial, which gives 5 mg/mL of blend and 2.5 mg/mL of each peptide. The calculator on this site works the same vial at 1, 2 and 3 mL, giving 10, 5 and 3.33 mg/mL of blend. More water means a larger draw for the same micrograms; it does not change what the vial contains.

Does a 10 mg blend vial contain 10 mg of each peptide?

Generally no. The labelled figure is the combined mass, so a 10 mg vial usually holds 10 mg of peptide in total, commonly 5 mg of CJC-1295 and 5 mg of ipamorelin. Some vials are filled at other ratios and some state only the total. The only authority on the split is the documentation that came with that vial, and every per-peptide figure on this page assumes a one-to-one fill that the reader has confirmed there.

How many units is 100 mcg of each peptide?

At a 5 mg plus 5 mg vial in 2 mL, each peptide is 2.5 mg/mL, so one U-100 unit carries 25 mcg of each and 100 mcg of each is 4 units; 200 mcg of each is 8 units and 300 mcg is 12. The same vial in 1 mL doubles the concentration and halves the units (2, 4 and 6), and in 3 mL they become 6, 12 and 18. A 5 mg blend vial in 2 mL is half as concentrated again: 8, 16 and 24 units.

What did the human trials of CJC-1295 and ipamorelin use?

Different molecules, routes and scales from the blend. Teichman and colleagues gave CJC-1295 with DAC subcutaneously to healthy adults and report it as well tolerated particularly at 30 or 60 mcg/kg, which is 2.25 to 4.5 mg for a 75 kg adult, given weekly or every two weeks. Ipamorelin's trials were intravenous: up to 140.45 nmol/kg over 15 minutes, and 0.03 mg/kg twice daily after bowel surgery. No trial has given the two together, by any route.

Is the CJC-1295 in a blend the DAC or the no-DAC form?

Usually the no-DAC form, which is the same GHRH fragment sold as Mod GRF 1-29, but both forms are on the research market and labelling is inconsistent. The distinction changes what the vial is, not the arithmetic: reconstitution depends only on the mass in the vial and the water added. It does change which human study, if any, the figure on the vial can be compared with, because the 2006 trial studied the DAC form alone.

When is the blend calculator needed instead of this page?

Whenever the two peptides are in separate vials, or a blended vial is filled at an uneven ratio. This page and the blend preset in the reconstitution calculator return the concentration of the blend as a whole and, at an assumed one-to-one split, of each half. The blend calculator takes each peptide's mass separately and returns the micrograms of each in any draw, which is the only correct method when the split is not equal.