Water volume for a 5 mg or 10 mg BPC-157 vial, the mcg/mL each volume gives and the U-100 units for the microgram amounts that circulate, sources dated.
BPC-157 is a synthetic 15-amino-acid fragment of a protein found in gastric juice, studied for decades in animals, mostly by one Croatian laboratory, and sold on the research market as a lyophilized powder in 5 mg and 10 mg vials. No product is approved anywhere, so there is no label, no approved concentration and no labelled water volume. Its amounts circulate in micrograms rather than milligrams, which makes the syringe arithmetic the whole question: a 5 mg vial holds twenty 250 mcg amounts, and the water decides whether each one is 5 units or 15.
This page reports that arithmetic for both vial sizes, sets the figures that circulate beside the few human reports that exist, and says where each came from. It recommends nothing. The BPC-157 calculator runs the same sums for any vial and volume; the BPC-157 dosage page reports the figures and their sources in full; the reconstitution hub indexes every compound.
The arithmetic in one line
Concentration in mcg/mL is the vial amount in micrograms divided by the water volume. Micrograms per unit is that figure divided by 100, because a U-100 syringe holds 100 units in 1 mL and each small notch on a standard 1 mL insulin syringe is 1 unit (MedlinePlus, "Giving an insulin injection", reviewed 2024-07-21). Units for an amount are the amount divided by the micrograms per unit.
Water volume per vial size
| Vial |
1 mL of water |
2 mL of water |
3 mL of water |
| 5 mg (5,000 mcg) |
5,000 mcg/mL, 50 mcg per unit |
2,500 mcg/mL, 25 mcg per unit |
1,667 mcg/mL, 16.7 mcg per unit |
| 10 mg (10,000 mcg) |
10,000 mcg/mL, 100 mcg per unit |
5,000 mcg/mL, 50 mcg per unit |
3,333 mcg/mL, 33.3 mcg per unit |
Units for the amounts that circulate
No label or controlled trial supplies an amount, so the columns are the figures most often cited on clinic and vendor pages on 2026-09-18, 250 to 500 mcg per day, with 750 and 1,000 mcg added because the arithmetic is the same and the pages sometimes go that high. They are converted here, not endorsed.
| Concentration |
250 mcg |
500 mcg |
750 mcg |
1,000 mcg |
| 1,667 mcg/mL |
15 units |
30 |
45 |
60 |
| 2,500 mcg/mL |
10 |
20 |
30 |
40 |
| 3,333 mcg/mL |
7.5 |
15 |
22.5 |
30 |
| 5,000 mcg/mL |
5 |
10 |
15 |
20 |
| 10,000 mcg/mL |
2.5 |
5 |
7.5 |
10 |
The pattern is the opposite of the milligram peptides on this site. A microgram amount never comes close to filling a syringe, so the concentrated end of the table is where the trouble is: at 10,000 mcg/mL, a 10 mg vial in 1 mL, 250 mcg is 2.5 units, which sits between two marks on a 1-unit syringe, and a one-unit misreading is 100 mcg, 40% of the amount. At 2,500 mcg/mL the same 250 mcg is 10 units and a one-unit misreading is 25 mcg, a tenth. More water gives a more readable draw; it never changes how many amounts the vial holds.
The 5 mg vial and the 10 mg vial
The two searched questions are what volume goes into a 5 mg vial and how a 10 mg vial is mixed, and the answers are the two rows above. A 5 mg vial in 1, 2 or 3 mL is 5,000, 2,500 or 1,667 mcg/mL, so 250 mcg is 5, 10 or 15 units; it holds twenty 250 mcg amounts at any volume. A 10 mg vial in 1, 2 or 3 mL is 10,000, 5,000 or 3,333 mcg/mL, so 250 mcg is 2.5, 5 or 7.5 units; it holds forty. A 10 mg vial in 2 mL and a 5 mg vial in 1 mL are the same solution, unit for unit.
Mixing steps, as labels describe them
No BPC-157 label describes mixing, because none exists. The steps that approved lyophilized peptide labels describe are consistent from product to product: the diluent is added to the vial, the vial is rolled gently rather than shaken, and the solution is inspected and used only if clear and free of particles (Egrifta SV label, DailyMed, revised 03/2024, read 2026-09-18). The general procedure, with supplies and order of operations, is Medibact's how to reconstitute peptides; this page does not repeat it.
Storage after mixing
No label states a storage period for a reconstituted BPC-157 vial, because no label exists; storage after mixing is covered by Medibact's how to store peptides, which this page links rather than expands.
The circulating figures carry no source. The human record is three small reports in one journal: a retrospective chart review of 16 knee-pain patients given intra-articular BPC-157, alone or with TB-500 (2021, PMID 34324435); a pilot in which 12 women with interstitial cystitis received 10 mg injected around the bladder inflammation in a single procedure (2024, PMID 39325560); and a two-person intravenous safety pilot at 10 mg on day one and 20 mg on day two (2025, PMID 40131143). None used a subcutaneous microgram amount, so none supports the 250 to 500 mcg figure, and none was controlled. Four registrations name the peptide on ClinicalTrials.gov, the largest a 120-participant phase 2 hamstring-strain trial recruiting since February 2026 (NCT07437547).
The regulatory line moved in 2026. FDA placed BPC-157 in Category 2 of its section 503A bulk-substance list, the category for substances that raise significant safety risks, on 2023-09-29. A Federal Register notice of 2026-04-16 scheduled BPC-157 for the Pharmacy Compounding Advisory Committee on 2026-07-23, with ulcerative colitis as the use FDA evaluated, and on that day the committee recommended that compounding pharmacies be allowed to prepare it, along with KPV, TB-500 and MOTS-c; the votes are not binding and FDA decides (STAT News, 2026-07-23, read 2026-09-18). None of that supplies a dose. The evidence, mechanism and sourcing belong to Medibact's BPC-157 guide, and the pairing with TB-500 that most searches attach to it is on this site's BPC-157 and TB-500 article.
Sources and dates
Opened 2026-09-18: DailyMed, BPC-157 search (no record); ClinicalTrials.gov v2 API (four registrations, listed by number); PubMed (220 records, none tagged as a clinical trial; the abstracts of PMIDs 34324435, 39325560 and 40131143 read in full); FDA's 503A category list dated 2023-09-29; Federal Register document 2026-07361 (2026-04-16); STAT News, 2026-07-23; MedlinePlus, "Giving an insulin injection" (reviewed 2024-07-21); the Egrifta SV label for the mixing description; clinic and vendor pages for the circulating figures. Vial sizes are the ones the calculator preset uses.
This page does not recommend a dose, a water volume, a syringe or a schedule, and it does not say what any figure means for a particular person. It reports what the sources state, converts micrograms into units, and says plainly that the circulating amounts have no source behind them.
