GLOW Reconstitution: Water, Concentration and Units

How much water a 70 mg GLOW vial takes, each component's concentration at 1 to 5 mL, units for a stated BPC-157 or GHK-Cu amount, and draws per vial.

Medibact Guides · Published 2026-09-27

Illustration: Two clear glass vials, one with water and one empty, on a white lab bench.
Illustration

GLOW is a pre-mixed vial of three peptides — GHK-Cu, BPC-157 and TB-500 — sold by its combined mass, usually 70 mg. Reconstituting it is the same act as for any vial, but the arithmetic runs three times, once per component, and the component a reader cares about decides how the reading is set. This page works the water volumes, the per-component concentrations and the readings for stated amounts. What the blend is and how it compares with KLOW are on this site's GLOW page and GLOW vs KLOW; the blend calculator works any other split.

The split every figure here assumes

Component Mass in a 70 mg vial Share by mass
GHK-Cu 50 mg 71.4%
BPC-157 10 mg 14.3%
TB-500 10 mg 14.3%

This is the split stated on retail listings read on 2026-09-23 and on both protocol pages read on 2026-09-27. It is a claim about products, not a standard: no monograph or regulator defines "GLOW", and only an assay shows what a given vial holds.

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Concentration at each water volume

Each component's concentration = its milligrams ÷ millilitres of water. One unit on a U-100 syringe is 0.01 mL.

Water GHK-Cu mg/mL BPC-157 mg/mL TB-500 mg/mL Per unit: GHK-Cu / BPC-157 / TB-500
1 mL 50 10 10 500 / 100 / 100 mcg
2 mL 25 5 5 250 / 50 / 50 mcg
2.5 mL 20 4 4 200 / 40 / 40 mcg
3 mL 16.7 3.33 3.33 167 / 33.3 / 33.3 mcg
4 mL 12.5 2.5 2.5 125 / 25 / 25 mcg
5 mL 10 2 2 100 / 20 / 20 mcg

The "total blend" figure some pages give — 23.3 mg/mL at 3 mL, 35 mg/mL at 2 mL — is the three columns added together. No component is present at that strength, so it cannot be used to read a syringe.

Setting the reading by one component

Every draw carries all three peptides in the fixed 5 : 1 : 1 ratio, so a reading chosen for one of them decides the other two.

Sized to a BPC-157 amount (units = amount in mcg × mL of water ÷ 100):

BPC-157 amount 1 mL 2 mL 3 mL 5 mL Also in the draw
250 mcg 2.5 5 7.5 12.5 1.25 mg GHK-Cu, 250 mcg TB-500
500 mcg 5 10 15 25 2.5 mg GHK-Cu, 500 mcg TB-500

Sized to a GHK-Cu amount (units = amount in mcg × mL of water ÷ 500):

GHK-Cu amount 1 mL 2 mL 3 mL 5 mL Also in the draw
1 mg 2 4 6 10 200 mcg each of BPC-157 and TB-500
2 mg 4 8 12 20 400 mcg each of BPC-157 and TB-500

Figures with a decimal fall between the 1-unit marks; at 1 mL the BPC-157 readings drop to 2.5 and 5 units, where one mark is 20–40% of the amount. More water spreads the same milligrams over more units, which is the only thing the volume changes. The per-compound figures in circulation for each peptide on its own are on the GHK-Cu, BPC-157 and TB-500 dosage pages; in GLOW they cannot be matched at once, because the blend fixes their ratio.

Two schedules, two volumes, nearly the same week

The two protocol pages read on 2026-09-27 state different water volumes and frequencies:

Page (date) Water Draw Frequency Per draw Per week
A (August 2026) 3 mL 10 units daily 1.67 mg GHK-Cu, 333 mcg BPC-157, 333 mcg TB-500 11.7 mg GHK-Cu, 2.33 mg each other
B (2026-07-29), first phase 2 mL 10 units 5 × a week 2.5 mg GHK-Cu, 500 mcg BPC-157, 500 mcg TB-500 12.5 mg GHK-Cu, 2.5 mg each other
B, later phase 2 mL 10 units 3 × a week same 7.5 mg GHK-Cu, 1.5 mg each other

Same "10 units", different milligrams per draw — 50% more on page B — yet weekly totals about 7% apart in the first phase. A reading quoted without its water volume is therefore not comparable between sources. Neither page cites a trial; no study of GLOW, or of any two of its components together, was found.

Draws per vial

Water Draws of 10 units Draws of 5 units
2 mL 20 40
2.5 mL 25 50
3 mL 30 60
5 mL 50 100

At 10 units a draw a vial gives 10 draws per millilitre, so page A's schedule uses a vial in about 30 days and page B's first phase in 4 weeks. The general mixing procedure is on Medibact's reconstitution how-to; the single-peptide arithmetic is on the GHK-Cu and BPC-157 reconstitution pages.

What the figures omit

The arithmetic assumes the vial holds exactly the stated milligrams of each peptide and that all three dissolve fully at the chosen volume; neither is shown without an assay. It says nothing about whether GLOW, or any amount of it, does anything in people. Nothing on this page is a dose, a volume to use or a schedule. The compound guides for GHK-Cu, BPC-157 and TB-500 are on the Medibact store.

Sources and dates

Opened 2026-09-27: two GLOW protocol pages (one dated August 2026, one dated 2026-07-29), quoted for the split, water, draw and frequency they state; this site's GLOW vs KLOW page for the retail-listing split read 2026-09-23 and its PubMed census of the components. Per-component concentrations, readings, weekly totals and draw counts are arithmetic by this site. Corrections go to the contact page.

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Frequently asked questions

How much bacteriostatic water goes in a 70 mg GLOW vial?

No label sets a volume; the protocol pages read on 2026-09-27 state 2 mL or 3 mL. With 2 mL, GHK-Cu is 25 mg/mL and BPC-157 and TB-500 are 5 mg/mL each; with 3 mL they are 16.7 and 3.33 mg/mL. The water changes how many units hold a given amount, not the ratio between the three peptides.

How many units of GLOW is 250 mcg of BPC-157?

At the 50/10/10 split: 5 units with 2 mL of water, 7.5 units with 3 mL, 2.5 units with 1 mL and 12.5 units with 5 mL. Each of those draws also holds 1.25 mg of GHK-Cu and 250 mcg of TB-500.

How much GHK-Cu is in 10 units of GLOW?

At the 50/10/10 split: 5 mg with 1 mL of water, 2.5 mg with 2 mL, 1.67 mg with 3 mL and 1 mg with 5 mL. BPC-157 and TB-500 are each one fifth of the GHK-Cu figure.

Can the total 70 mg be divided by the water to get a dose?

It gives a number, 23.3 mg/mL at 3 mL, but not a useful one: no component is present at that concentration. Each component's concentration is its own milligrams divided by the water, so the total only restates the three figures added together.

How long does a GLOW vial last?

It depends on the water and the draw. At 10 units a draw there are 10 draws per millilitre of water: 20 at 2 mL and 30 at 3 mL. On the two schedules protocol pages state, that is about 30 days (3 mL, daily) and 4 weeks (2 mL, five times a week).