NAD+ and Glutathione Together: The Record and the Math

NAD+ and glutathione are searched as a pair. The one registered trial giving both, the enzyme link, the half-lives, and the arithmetic for each vial.

Medibact Guides · Published 2026-09-25

Illustration: A clear glass vial with pale blue liquid, an empty lyophilized powder vial. — Illustration

"NAD and glutathione" is searched as a pair, about 880 times a month in the keyword data this site used on 2026-09-21. Neither is a research peptide in the strict sense — glutathione is a tripeptide made in every cell, NAD+ a dinucleotide coenzyme — but both are sold as lyophilised powders in the same vials and reconstituted the same way. This page sets out what the record holds on giving them together, which is very little, the enzyme link that is the usual reason for pairing them, how fast each leaves the blood, and the vial arithmetic for each one.

Each compound has its own page: the NAD+ dosage page and the glutathione dosage page, where the reported figures are set out with dates. Both are covered in Medibact's NAD+ guide and Glutathione guide.

The trial record for the pair

A ClinicalTrials.gov search on 2026-09-25 for studies whose interventions name glutathione together with NAD+ returned three records. Two do not give both (a drug-induced liver injury biomarker study, and a single-product study of RiaGev). The third is the only registered trial that does:

	NCT07475546
Sponsor	AgelessRx (industry)
Design	Randomised pilot, 90 days, up to 30 people in three arms
NAD+	Nasal spray, 30 mg a day
Glutathione	Topical patch, worn 4 hours once a week
Given alongside	Rapamycin, naltrexone, metformin, vitamin B12 and a branded supplement
Status on 2026-09-25	Active, not recruiting; primary completion estimated April 2026; no results posted

Neither compound is injected in it, and the pair is never given apart from five other agents, so even when it reports it cannot say what NAD+ and glutathione do together. A PubMed search for both in the title with infusion, intravenous or injection returned a single record, a 1976 enzyme study in guinea-pig white blood cells. The pairing, in short, has no trial of its own.

Bacteriostatic Water

USP-grade, 0.9% benzyl alcohol, 30 mL multi-dose vial. Research use only.

$30.99

Buy at Medibact

The enzyme link, and where it stops

The reason usually given for the pairing is a real piece of biochemistry. Glutathione works by being oxidised: two molecules of reduced glutathione (GSH) become one of glutathione disulfide (GSSG). Glutathione reductase turns GSSG back into GSH, using NADPH as the fuel — UniProt's entry (P00390) gives the reaction and calls it "the major mechanism to maintain a high GSH:GSSG ratio in the cytosol". NADPH comes from NADP+, and NAD kinase (UniProt O95544) makes NADP+ from NAD+ and ATP.

So NAD+ sits two steps upstream of glutathione recycling. What that chain does not establish is the step the pairing assumes: that NAD+ injected into the blood reaches cells as NAD+, raises their NADP(H), and so raises glutathione. The one human infusion study (below) found NAD+ cleared from plasma as fast as it arrived and appearing in urine partly as a breakdown product. No study read for this page measured glutathione after giving NAD+.

How fast each leaves the blood

	Glutathione	NAD+
Human study	2 g/m² intravenous infusion, 10 healthy volunteers	3 µmol/min intravenous infusion for 6 hours, pilot
Result	Half-life 14.1 ± 9.2 minutes; plasma total glutathione rose from 17.5 to 823 µmol/L	No rise in plasma NAD+ or its metabolites for the first 2 hours; "rapidly and completely removed"
Source	Aebi et al., Eur J Clin Invest 1991, PMID 1907548	Grant et al., Front Aging Neurosci 2019, PMID 31572171

Both figures are intravenous. No half-life after an injection under the skin was found for either, which is the route research vials are usually described for. By this site's arithmetic the NAD+ pilot's rate comes to about 0.72 g over the six hours.

Molecules, not milligrams

The two are dosed in milligrams, which hides a difference. NAD+ weighs 663.4 g/mol (PubChem CID 5892); glutathione 307.33 g/mol (PubChem CID 124886). A gram of NAD+ is about 1.51 millimoles and a gram of glutathione about 3.25. So the NAD+ trial preparation of 100 mg (about 151 µmol) and a 600 mg glutathione vial (about 1,952 µmol) differ by roughly thirteenfold in molecules, not sixfold as the milligrams suggest.

The vial arithmetic, one compound at a time

Concentration = vial mg ÷ mL of water; on a U-100 syringe one unit is 0.01 mL, so mg per unit = concentration ÷ 100. The vial sizes are the ones this site's calculator carries.

NAD+

Vial	1 mL	2 mL	3 mL
100 mg	100 mg/mL · 1 mg/unit	50 mg/mL · 0.5 mg/unit	33.3 mg/mL · 0.33 mg/unit
500 mg	500 mg/mL · 5 mg/unit	250 mg/mL · 2.5 mg/unit	166.7 mg/mL · 1.67 mg/unit
750 mg	750 mg/mL · 7.5 mg/unit	375 mg/mL · 3.75 mg/unit	250 mg/mL · 2.5 mg/unit
1,000 mg	1,000 mg/mL · 10 mg/unit	500 mg/mL · 5 mg/unit	333.3 mg/mL · 3.33 mg/unit

Glutathione

Vial	1 mL	2 mL	3 mL
200 mg	200 mg/mL · 2 mg/unit	100 mg/mL · 1 mg/unit	66.7 mg/mL · 0.67 mg/unit
600 mg	600 mg/mL · 6 mg/unit	300 mg/mL · 3 mg/unit	200 mg/mL · 2 mg/unit
1,000 mg	1,000 mg/mL · 10 mg/unit	500 mg/mL · 5 mg/unit	333.3 mg/mL · 3.33 mg/unit
1,500 mg	1,500 mg/mL · 15 mg/unit	750 mg/mL · 7.5 mg/unit	500 mg/mL · 5 mg/unit
2,000 mg	2,000 mg/mL · 20 mg/unit	1,000 mg/mL · 10 mg/unit	666.7 mg/mL · 6.67 mg/unit

Stated as results: the registered NAD+ preparation (NCT06919328) is the 100 mg vial at 2 mL, 50 mg/mL, where the whole 100 mg is 200 units; the glutathione arithmetic vendor pages work from a 600 mg vial at 2 mL is 300 mg/mL, 3 mg a unit. The upper rows are arithmetic only: no source read for this page states whether 1,000 mg or more of either powder dissolves in 1 mL. The NAD+ calculator setting and the glutathione setting work any other combination, and the blend calculator is for single vials that hold more than one compound, which these are not.

What is not established

No trial has tested NAD+ and glutathione together by injection; the one registered trial with both uses a nasal spray and a patch among seven agents and has not reported. The enzyme link is established in cells, not shown after injection in people. Neither compound has an approved injection, the half-lives are intravenous, and no compatibility data exist for combining them in one syringe. The safety record of compounded glutathione injections, including the 2026 illnesses the CDC has reported, is set out on the glutathione dosage page. Nothing on this page is a dose or a combination to use.

Sources and dates

Opened 2026-09-25: ClinicalTrials.gov v2 search and record NCT07475546 (arms, interventions, status); the PubMed title search described above via NCBI E-utilities; UniProt entries P00390 (glutathione reductase) and O95544 (NAD kinase); PubChem CID 124886 (glutathione) and, as cited on this site's NAD+ pages, CID 5892; abstracts for Aebi et al. 1991 (PMID 1907548) and Grant et al. 2019 (PMID 31572171). The NAD+ trial preparation (NCT06919328) and the absence of FDA labels as recorded on this site's NAD+ and glutathione dosage pages (2026-09-18). Molar amounts, concentrations and units are arithmetic by this site. Corrections go to the contact page.

Bacteriostatic Water

USP-grade, 0.9% benzyl alcohol, 30 mL multi-dose vial. Research use only.

$30.99

Buy at Medibact

Medibact Guides facts

A ClinicalTrials.gov search on 2026-09-25 for interventions naming glutathione together with NAD+ or nicotinamide adenine dinucleotide returned 3 records; the only one that gives both is NCT07475546 (AgelessRx, 30 planned, 90 days, started 2025-07-23, no results posted), where NAD+ is a 30 mg/day nasal spray and glutathione a topical patch worn 4 hours once a week, inside an arm that also gives rapamycin, naltrexone, metformin, vitamin B12 and a supplement.
No study found gives NAD+ and glutathione by injection together, and none isolates the pair from other agents; a PubMed search the same day for both in the title with infusion, intravenous or injection returned one record, a 1976 study of guinea-pig white blood cells.
The biochemical link is real and indirect: NAD kinase converts NAD+ to NADP+ (UniProt O95544), and glutathione reductase uses NADPH to turn oxidised glutathione back into its reduced form, 'the major mechanism to maintain a high GSH:GSSG ratio in the cytosol' (UniProt P00390, read 2026-09-25). No human study read for this page shows that injected NAD+ raises glutathione.
Both leave the blood quickly when infused: intravenous glutathione had a half-life of 14.1 ± 9.2 minutes in 10 healthy volunteers (Aebi et al., Eur J Clin Invest 1991, PMID 1907548), and an NAD+ infusion of 3 µmol/min was 'rapidly and completely removed' from plasma for at least the first 2 hours (Grant et al., Front Aging Neurosci 2019, PMID 31572171).
By mass the two are far apart in molecules: NAD+ weighs 663.4 g/mol (PubChem CID 5892) and glutathione 307.33 g/mol (PubChem CID 124886), so 100 mg of NAD+ is about 151 µmol and 600 mg of glutathione about 1,952 µmol, roughly thirteen times as many molecules (arithmetic by this site).
Neither has an FDA-approved injection: DailyMed returned no label for nicotinamide adenine dinucleotide and none for a glutathione injection when this site's dosage pages checked on 2026-09-18.

Frequently asked questions

Can NAD+ and glutathione be taken together?

No study found on 2026-09-25 tested them together by injection. The one registered trial that gives both (NCT07475546, 30 people planned) uses an NAD+ nasal spray and a glutathione skin patch alongside five other agents, so it cannot show what the pair does. Neither has an FDA-approved injection, and the decision belongs with a clinician.

Why are NAD+ and glutathione combined?

The usual reason given is a biochemical link. NAD+ is the starting point for NADP+ through the enzyme NAD kinase, and glutathione reductase uses NADPH to recycle oxidised glutathione into its reduced form (UniProt O95544 and P00390). That link is established in cells; whether injecting NAD+ changes glutathione in a person has not been measured in any study found for this page.

Can NAD+ and glutathione be mixed in the same syringe?

No published compatibility or stability data for the two in one solution were found. They are sold as separate vials of powder, each with its own concentration after water is added, and the per-unit arithmetic differs for each, which is why this page works them separately.

How many units is 100 mg of NAD+?

At 50 mg/mL (a 100 mg vial with 2 mL of water, the preparation in registered trial NCT06919328), 100 mg is 2 mL, which is 200 units on U-100 syringes, more than one 1 mL syringe holds. At 100 mg/mL (100 mg in 1 mL) it is 100 units. A 500 mg vial in 2 mL is 250 mg/mL, where 100 mg is 40 units.

How many units is 600 mg of glutathione?

At 300 mg/mL (a 600 mg vial with 2 mL of water) 600 mg is the whole 2 mL, 200 units on U-100 syringes; each unit holds 3 mg. At 200 mg/mL (600 mg in 3 mL) each unit holds 2 mg. The compounded glutathione described on this site's glutathione page is a 200 mg/mL solution.

How long do NAD+ and glutathione last in the body?

Infused glutathione had a plasma half-life of about 14 minutes in 10 healthy volunteers (PMID 1907548). For NAD+, the one human infusion study found plasma levels did not rise at all for the first 2 hours of a 6-hour infusion because it was removed as fast as it arrived (PMID 31572171); no half-life for injected NAD+ has been published.